DNA

Part:BBa_K4051014:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-05)


PCRA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1318
    Illegal BamHI site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2816
    Illegal AgeI site found at 1127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised, primers designed


Source

Dechloromonas (Azospira) suillum locus Dsui_0145

Genbank Accession: CP003153.1

Generic Histag used

Generic Secretion Signal from Gaussian luciferase


References